Seo u lokelang ho se ela hloko ha u sebelisaBOD analyzer:
1. Ho itokisetsa pele ho liteko
1. Bulela phepelo ea motlakase ea biochemical incubator lihora tse 8 pele ho teko, 'me u laole mocheso hore o sebetse ka tloaelo ho 20°C.
2. Kenya metsi a hlapollo ea liteko, metsi a ente le metsi a hlapollotsoeng ka har'a incubator 'me u li boloke li le mochesong o sa fetoheng hore li sebelisoe hamorao.
2. Tšebeliso ea pele ea metsi
1. Ha boleng ba pH ba sampole ea metsi bo se pakeng tsa 6.5 le 7.5; pele etsa tlhahlobo e arohaneng ho fumana hore na molumo o hlokahalang oa hydrochloric acid (5.10) kapa sodium hydroxide solution (5.9), ebe o fokotsa sampole, ho sa tsotelehe hore na ho na le pula. Ha acidity kapa alkalinity ea sampole ea metsi e phahame haholo, alkali kapa asiti e phahameng haholo e ka sebelisoa bakeng sa ho se nke lehlakore, ho netefatsa hore chelete ha e ka tlase ho 0.5% ea molumo oa sampole ea metsi.
2. Bakeng sa lisebelisoa tsa metsi tse nang le chlorine e nyenyane ea mahala, chlorine e sa lefelloeng hangata e tla nyamela ka mor'a hore e tlohe lihora tse 1-2. Bakeng sa disampole tsa metsi moo chlorine ea mahala e ke keng ea nyamela ka nako e khuts'oane, ho ka eketsoa tekanyo e loketseng ea sodium sulfite ho tlosa chlorine ea mahala.
3. Mehlala ea metsi e bokeletsoeng metsing a nang le mocheso o tlase oa metsi kapa matša a eutrophic a lokela ho futhumatsoa ka potlako ho isa ho 20 ° C ho leleka oksijene e qhibilihisitsoeng ka bongata metsing. Ho seng joalo, liphetho tsa tlhahlobo li tla ba tlase.
Ha u nka lisampole ho tsoa libakeng tsa metsi tse nang le mocheso o phahameng oa metsi kapa libaka tsa ho ntša metsi a litšila, li lokela ho pholile ka potlako hoo e ka bang 20 ° C, ho seng joalo liphello tsa tlhahlobo li tla ba tse phahameng.
4. Haeba sampuli ea metsi e lokelang ho hlahlojoa e se na likokoana-hloko kapa ts'ebetso e sa lekaneng ea likokoana-hloko, sampuli e tlameha ho kenngoa. Joalo ka mefuta e latelang ea metsi a litšila a indasteri:
a. Metsi a litšila a indasteri a so kang a hloekisoa ka biochemical;
b. Mocheso o phahameng le khatello e phahameng kapa metsi a litšila a sterilized, tlhokomelo e khethehileng e lokela ho lefshoa ho metsi a litšila a tsoang indastering ea ho lokisa lijo le likhoerekhoere tsa malapeng tse tsoang lipetlele;
c. Metsi a litšila a indasteri a nang le asiti e matla le a alkaline;
d. Metsi a litšila a indasteri a nang le boleng bo phahameng ba BOD5;
e. Metsi a litšila a indasteri a nang le lintho tse chefo joalo ka koporo, zinki, lead, arsenic, cadmium, chromium, cyanide, jj.
Metsi a litšila a ka holimo a indasteri a hloka ho hloekisoa ka likokoana-hloko tse lekaneng. Mehloli ea li-microorganisms ke tse latelang:
(1) The supernatant ea likhoerekhoere tse hloekileng tsa ka tlung tse sa tsoakoang tse behiloeng ho 20°C bakeng sa lihora tse 24 ho isa ho tse 36;
(2) Mokelikeli o fumanoeng ka ho sefa sampuli ka pampiri ea ho hloekisa ka mor'a hore tlhahlobo e fetileng e phethoe. Mokelikeli ona o ka bolokoa ho 20 ℃ khoeli e le 'ngoe;
(3) Matla a tsoang litsing tsa likhoerekhoere;
(4) Metsi a noka kapa a letša a nang le likhoerekhoere tsa litoropo;
(5) Mefuta ea baktheria e fanoang ka sesebelisoa. Beha boima ba 0.2g ea likokoana-hloko tsa baktheria, u li tšele ka 100ml ea metsi a hloekileng, u tsose ka ho tsoelang pele ho fihlela likhama li qhalakanngoa, li kenye ka har'a incubator ka 20 ° C 'me u e tlohelle lihora tse 24-48, ebe u nka supernatant.
Nako ea poso: Jan-24-2024
